Journal: Frontiers in Immunology
Article Title: High-mobility group protein B1 derived mutant peptide mB Box-97 inhibits the formation of neutrophil extracellular traps
doi: 10.3389/fimmu.2025.1565252
Figure Lengend Snippet: Ca 2+ ionophore-mediated NETosis was partially inhibited by mB Box-97 syn . CLSM images of the NETs induced using Ca 2+ ionophore A23187 with or without mB Box-97 syn or B Box-97 are shown where blue represents DNA), green represents plasma membrane, and red represents NE (upper panel) or MPO (lower panel). n = 3 independent experiments, 63x magnification, scale bar 50 µm.
Article Snippet: Cells were then stimulated with 100 nM PMA, heat-inactivated NTHI (MOI=10), or 1 μM Ca2+ ionophore A23187 (Millipore Sigma, C7522) with or without mB Box-97 syn or B Box-97 (200 nM) and incubated for 3-4 hours at 37°C.
Techniques: Clinical Proteomics, Membrane